Atg9a abcam
WebThis western blot data was generated using the same anti-ATG9A clone (EPR2450(2)] in a different buffer formulation (cat# ab108338). Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: ATG9A knockout HAP1 cell lysate … WebAug 20, 2015 · After fixation with ice-cold methanol, and blocking with 10% fetal bovine serum in PBS, the cells were incubated with anti-Annexin A2 (Santa Cruz Biotechnology; 3D5) and anti-ATG9A (Abcam; EPR2450 ...
Atg9a abcam
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WebSep 27, 2024 · Our study uncovers ATG9A trafficking as a ubiquitous function of the AP-4 pathway. ... (ab6556, Abcam), chicken anti-GFP 1:500 for IF (ab13970, Abcam), mouse anti-HA 1:2000 for WB and IF (16B12 ... WebSep 14, 2024 · Knockdown of either PIK3C2A or ATG9A/B, but not PI3KC3, results in an accumulation of transferrin-positive clathrin coated vesicles and RAB11-positive vesicles at the recycling endosome. Taken together, these results support a role for PI3K-C2α in the proper maturation of endosomes, and suggest that PI3K-C2α may be a critical node …
WebMammalian Ddrgk1 was shown to be indispensable for the stability of endogenous Atg9A protein in mouse embryonic fibroblast (MEF) cells. Taken together, our findings might have important implications for neurodegenerative diseases in mammals. ... 1:2000) and anti-Atg9A (Abcam, ab108338, 1:2000), and quantified by image J. Immunoprecipitation.
WebAp4b1-knockout mouse model of hereditary spastic paraplegia type 47 displays motor dysfunction, aberrant brain morphology and ATG9A mislocalization. View PDF. In Brain … WebAug 18, 2024 · As shown in Fig. 6, there was a trend of increases in levels of Ulk1, Beclin-1, Atg9a, Atg4b, and Bnip3 mRNAs in both FoxO3 +/+ and FoxO3 −/− mouse kidneys with a longer duration of obstruction. However, these mRNAs were at significantly higher levels in FoxO3 +/+ compared with FoxO3 −/− mouse kidneys at 7 days post-UUO.
WebApr 7, 2024 · Next, the slides were uploaded to the autostainer according to the instructions from the manufacturer. The primary antibodies used here include AUP1 (Atlas antibodies #HPA007674), P53 (cell signaling # 2524), and KI67 (Abcam #ab15580). The secondary antibodies used Roche Diagnostics OptiView DAB IHC Detection Kit.
WebJul 12, 2024 · The integral membrane protein ATG9A plays a key role in autophagy. It displays a broad intracellular distribution and is present in numerous compartments, … distance around lake merritt oakland caWebAug 23, 2024 · ATG9A Is Overexpressed in Triple Negative Breast Cancer and Its In Vitro Extinction Leads to the Inhibition of Pro-Cancer Phenotypes. Cells. 2024;7: pubmed … distance around pool tableWebApr 12, 2024 · ATG5 (Abcam, ab108327) was specially used in colocalization, ATG9A (Abcam, ab108338) was specially used in immunofluorescence (IF) staining and immunohistochemistry (IHC) analysis and TIMP3 (Proteintech, 10858-1-AP) was specially used in IHC analysis. 2.2. Cell Culture. cpprq stock price todayWebApr 12, 2024 · ATG9A (Abcam, ab108338) was specially used in immuno- fl uorescence (IF) staining and immunohistochemistry (IHC) analysis and TIMP3 (Proteintech, 10858-1-AP) was specially distance around the city of jerichoWebATG9A is a multispanning membrane protein essential for autophagy. Normally resident in Golgi membranes and endosomes, during amino acid starvation, ATG9A traffics to sites of autophagosome formation. ATG9A is not incorporated into autophagosomes but is proposed to supply so-far-unidentified proteins and lipids to the autophagosome. distance around flat rock park columbus gaWebAbcam hamster monoclonal anti atg9a antibody Hamster Monoclonal Anti Atg9a Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. distance around national mallWebCompany: Abcam. Antibody: ATG9A . Catalog number: ab108338. Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to a region within human … distance around soccer field