Phic31 integrase system
WebThe phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two 34 … WebThe phiC31 integrase is a sequence-specific recombinase encoded within the genome of the bacteriophage phiC31. The phiC31 integrase mediates recombination between two …
Phic31 integrase system
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WebJump-In™ Fast Gateway® System allows you to generate stable clones in just two weeks by introducing your genetic material into your cell background using PhiC31 integrase to … WebMar 4, 2016 · Use of phiC31 Integrase for Constructing Transgenic Organisms One application of phage integrases that has been popular is the use of phiC31 integrase to place transgenes into the genome to construct transgenic organisms. Both pseudo att sites and authentic att sites have been used in this regard.
WebOct 17, 2005 · The phiBT1 phage integrase is an enzyme that catalyses the efficient recombination between unique sequences in the phage and bacterial genomes, leading to the site-specific integration of the former into the latter in a unidirectional manner. WebMay 22, 2024 · Mammalian system plasmids. For the integrase expression vectors, Ints 2, 4, 5, 7, 9, 13, phiC31, and Bxb1 were placed under the control of the ubiquitin promoter and β-globin poly(A) signal ...
WebA method of controlling a genetically-modified plant, comprising (a) providing a genetically-modified plant, whereby cells of said genetically-modified plant contain a heterologous nucleic acid and whereby said genetically-modified plant is inactive with regard to a cellular process of interest, (b) switching on said cellular process of interest by directly … WebSimply co-transfect with the PhiC31 Integrase Expression Plasmid (Cat.# FC200PA-1) and select for integrants using puromycin resistance and imaging for RFP fluorescence. The bidirectional promoter configuration takes advantage of the natural bidirectional activity of the PGK promoter.
WebThe phiC31 integrase system efficiently integrates plasmid DNA carrying a transgene and an attB site into a limited number of endogenous pseudo attP sites in mammalian genomes, leading to robust ...
Web• Developed phiC31 integrase system in Hydra to optimize transgenesis via site-specific recombination. • Designed DNA constructs for microinjections. • Mentored undergraduate students and ... christmas tree push in clipsWebFeb 28, 2014 · PhiC31 integrase is a DNA recombinase derived from Streptomyces phage φC31 1. This enzyme can mediate recombination between two sequences, namely, attB … christmas tree pull apart bread recipesgetphysicalnumberofcells getlastcellnumWebMay 7, 2013 · We showed in our previous paper that phiC31 integrase works very efficiently in silkworm embryos using an extra chromosomal cassette exchange reaction system; recombination between the attP and attB sites of the plasmid occurred with a high frequency in the silkworm embryos when phiC31-integrase mRNA was supplied by injection and no … christmas tree push fasteners plasticWebThe phiC31 integrase system represents a novel technology that opens up new possibilities for gene therapy. The phiC31 integrase can integrate introduced plasmid DNA into … christmas tree quilt block pattern freeWebJun 17, 2010 · In addition to the widely used Cre and Flp recombinases, the phiC31 integrase system from Streptomyces phage may also be used for these genetic manipulations in eukaryotic cells. Unlike Cre and Flp, phiC31 recognizes two heterotypic sites, attB and attP, for recombination. get physical exam near meWebJul 29, 2012 · The phiC31 integrase system. is thought to be more efficient than the FLP recombinase for. the construction of transgenic insects because it has been. successfully applied to diverse organisms ... christmas tree quotes clip art